Search results for "Cytological Techniques"

showing 10 items of 15 documents

Reduced secretion of proinflammatory cytokines of monosodium urate crystal‐stimulated monocytes in chronic renal failure: an explanation for infreque…

2000

In gouty arthritis, monosodium urate (MSU) crystals interact with monocytes and neutrophils to produce inflammatory reactions associated with acute synovitis. In patients with end-stage renal disease (ESRD), gouty arthritis is a rare condition despite often severe hyperuricaemia. We wondered whether differences in the secretion of proinflammatory cytokines by MSU crystal-stimulated monocytes might be one explanation for the low incidence of gouty arthritis in patients with ESRD compared with healthy controls.Thirteen patients with ESRD on intermittent haemodialysis treatment, six patients with chronic renal failure not yet on dialysis, and 15 age- and sex-matched healthy controls were exami…

AdultLipopolysaccharidesMalemusculoskeletal diseasescongenital hereditary and neonatal diseases and abnormalitiesmedicine.medical_specialtyGoutmedicine.medical_treatmentCytological TechniquesInflammationurologic and male genital diseasesProinflammatory cytokinechemistry.chemical_compoundInternal medicinemedicineHumansAgedAged 80 and overTransplantationInterleukin-6Tumor Necrosis Factor-alphabusiness.industryIncidencenutritional and metabolic diseasesMiddle Agedmedicine.diseasePathophysiologyUric AcidGoutEndocrinologyCytokinechemistryNephrologyAcute SynovitisImmunologyCytokinesKidney Failure ChronicUric acidFemaleInflammation Mediatorsmedicine.symptomCrystallizationbusinessInterleukin-1Kidney diseaseNephrology Dialysis Transplantation
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High resolution analysis and differential condensation in RBA-banded human chromosomes.

1981

Human prophase, premetaphase, and mid-metaphase chromosomes are prepared and analyzed using the thymidine cell synchronization technique and R-banding patterns (RBA). Haploid sets with 700–1000 bands can be demonstrated. Sequences of chromosomes of different degrees of condensation are helpful for a better understanding and classification of regions of extended chromosomes. A considerable variation in the condensation of parts of homologous chromosomes is reflected in the variability of the arm ratio. This differential condensation of chromosomes is entirely effected by variation of the degree of condensation in AT rich interbands and can be attributed to the degree of labeling by BrdU.

High resolution analysisGeneticsCondensationCytological TechniquesGenetic VariationBiologyProphaseAcridine OrangeChromosome BandingProphaseBromodeoxyuridineGeneticsHomologous chromosomeBiophysicsChromosomes HumanHumansPloidyCell synchronizationGenetics (clinical)MetaphaseHuman genetics
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Current trends in biocompatibility testing

1998

Biocompatibility remains the central theme for biomaterials applications in medicine. It is generally accepted that this term means not only absence of a cytotoxic effect but also positive effects in the sense of biofunctionality, i.e. promotion of biological processes which further the intended aim of the application of a biomaterial. The national and international standards for testing regimes represent a lowest common denominator for such applications and do not necessarily ensure that optimal function will be achieved. The authors' thesis is that biocompatibility testing has scope for extensive development with respect to biofunctionality. The present paper reviews current trends in the…

In Vitro TechniquesBiocompatibilitymedia_common.quotation_subjectCytological TechniquesBiocompatible MaterialsNanotechnologyIn Vitro TechniquesBiologyOrgan development03 medical and health sciences0302 clinical medicineMaterials TestingCell AdhesionMedical Laboratory ScienceAnimalsHumansLowest common denominatorFunction (engineering)Cells Culturedmedia_commonScope (project management)Mechanical EngineeringBiocompatibility TestingReproducibility of ResultsGeneral MedicineCytotoxicity Tests ImmunologicCritical appraisalRisk analysis (engineering)030220 oncology & carcinogenesisStress MechanicalRheology030217 neurology & neurosurgeryForecastingProceedings of the Institution of Mechanical Engineers, Part H: Journal of Engineering in Medicine
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Multicolor fate mapping of Langerhans cell homeostasis

2013

The adult epidermal Langerhans cell network is formed by adjacent proliferative units composed of dividing cells and their terminally differentiated daughter cells.

Langerhans cellCell divisionImmunologyPopulationCellCytological TechniquesColorMice TransgenicBiology03 medical and health sciencesMice0302 clinical medicineImmune systemImaging Three-DimensionalFate mappingmedicineImmunology and AllergyAnimalsHomeostasisCell Lineageeducation030304 developmental biologyInflammation0303 health scienceseducation.field_of_studyEpidermis (botany)integumentary systemBrief Definitive ReportCell biologyMice Inbred C57BLmedicine.anatomical_structure030220 oncology & carcinogenesisLangerhans CellsImmunologyStem cellJournal of Experimental Medicine
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Standardization of reagents and methods used in cytological and histological practice with emphasis on dyes, stains and chromogenic reagents

1994

The need for the standardization of reagents and methods used in the histology laboratory is demonstrated. After definitions of dyes, stains, and chromogenic reagents, existing standards and standards organizations are discussed. This is followed by practical instructions on how to standardize dyes and stains through the preparation of reference materials and the development of chromatographic methods. An overview is presented of the problems concerned with standardization of the Romanowsky-Giemsa stain for cytological and histological application. Finally, the problem of how to convince routine dye and stain users of the need for standardization in their histology laboratories is discussed.

Quality ControlChromatographyStaining and LabelingStandardizationChemistryChromogenicCytological TechniquesHistological TechniquesCell BiologyReference StandardsStainStainingInvestigation methodsChromogenic CompoundsAnimalsHumansAnatomyColoring AgentsReference standardsThe Histochemical Journal
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White blood cell counts as risk markers of developing metabolic syndrome and its components in the Predimed study.

2013

Journal Article; Multicenter Study; Randomized Controlled Trial; Research Support, Non-U.S. Gov't; TRIAL REGISTRATION Controlled-Trials.comISRCTN35739639. BACKGROUND The Metabolic Syndrome (MetS) is a cluster of metabolic abnormalities that includes hyperglucemia, hypertension, dyslipidemia and central obesity, conferring an increased risk of cardiovascular disease. The white blood cell (WBC) count has been proposed as a marker for predicting cardiovascular risk. However, few prospective studies have evaluated the relationship between WBC subtypes and risk of MetS. METHODS Participants were recruited from seven PREDIMED study centers. Both a baseline cross-sectional (n = 4,377) and a prospe…

MaleSíndrome metabòlicaMediterranean dietNeutrophilsEpidemiologyEstudios transversalesMetabolic disorders:Named Groups::Persons::Age Groups::Adult::Aged::Aged 80 and over [Medical Subject Headings]Disease:Named Groups::Persons::Age Groups::Adult::Middle Aged [Medical Subject Headings]Diet Mediterranean:Analytical Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Epidemiologic Methods::Statistics as Topic::Probability::Risk::Risk Factors [Medical Subject Headings]:Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans [Medical Subject Headings]Leukocyte CountLymphocitesRisk FactorsBlood plasmaPathologyMedicineProspective StudiesProspective cohort studyAged 80 and overMetabolic SyndromeClinical ChemistryMultidisciplinary:Analytical Diagnostic and Therapeutic Techniques and Equipment::Therapeutics::Nutrition Therapy::Diet Therapy::Diet Mediterranean [Medical Subject Headings]QRMiddle AgedMetabolic syndromeClinical Laboratory Sciences1932-6203:Analytical Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Epidemiologic Methods::Epidemiologic Study Characteristics as Topic::Epidemiologic Studies::Cross-Sectional Studies [Medical Subject Headings]medicine.anatomical_structureCholesterolCardiovascular diseasesCardiovascular DiseasesMedicineWhite blood cellsFemalePublic HealthEnfermedades cardiovascularesResearch Articlemedicine.medical_specialty:Analytical Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Epidemiologic Methods::Epidemiologic Study Characteristics as Topic::Epidemiologic Studies::Cohort Studies::Longitudinal Studies::Prospective Studies [Medical Subject Headings]Recuento de linfocitosScience:Check Tags::Male [Medical Subject Headings]Mediterranean cookingDiagnostic MedicineInternal medicineWhite blood cell:Analytical Diagnostic and Therapeutic Techniques and Equipment::Diagnosis::Diagnostic Techniques and Procedures::Clinical Laboratory Techniques::Cytological Techniques::Cell Count::Blood Cell Count::Leukocyte Count::Lymphocyte Count [Medical Subject Headings]Cuina mediterràniaHumansLymphocyte CountObesity:Named Groups::Persons::Age Groups::Adult::Aged [Medical Subject Headings]:Diseases::Cardiovascular Diseases [Medical Subject Headings]Cardiovascular Disease EpidemiologyAged:Diseases::Nutritional and Metabolic Diseases::Metabolic Diseases::Metabolic Syndrome X [Medical Subject Headings]business.industryMalalties cardiovascularsRecuento de leucocitosSíndrome X metabólicomedicine.diseaseObesityLong-Term CareDieta mediterránea:Analytical Diagnostic and Therapeutic Techniques and Equipment::Diagnosis::Diagnostic Techniques and Procedures::Clinical Laboratory Techniques::Cytological Techniques::Cell Count::Blood Cell Count::Leukocyte Count [Medical Subject Headings]Biomarker EpidemiologyCross-Sectional Studies:Check Tags::Female [Medical Subject Headings]GeriatricsImmunologyClinical ImmunologyPreventive MedicineMetabolic syndrome:Anatomy::Cells::Blood Cells::Leukocytes::Granulocytes::Neutrophils [Medical Subject Headings]businessDyslipidemiaBiomarkersGeneral Pathology
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Safety and immunomodulatory effects of three probiotic strains isolated from the feces of breast-fed infants in healthy adults: SETOPROB study.

2013

We previously described the isolation and characterization of three probiotic strains from the feces of exclusively breast-fed newborn infants: Lactobacillus paracasei CNCM I-4034, Bifidobacterium breve CNCM I-4035 and Lactobacillus rhamnosus CNCM I-4036. These strains were shown to adhere to intestinal mucus in vitro, to be sensitive to antibiotics and to resist biliary salts and low pH. In the present study, a multicenter, randomized, double-blind, placebo-controlled trial with 100 healthy volunteers in three Spanish cities was carried out to evaluate the tolerance, safety, gut colonization and immunomodulatory effects of these three probiotics. Volunteers underwent a 15-day washout perio…

ARN Bacterianoved/biology.organism_classification_rank.speciesPhysiologylcsh:Medicine:Phenomena and Processes::Biological Phenomena::Ecological and Environmental Phenomena::Environment::Ecosystem::Biodiversity::Biota::Microbiota [Medical Subject Headings]law.inventionFecesProbioticAntibioticslawLactobacillus:Phenomena and Processes::Physiological Phenomena::Nutritional Physiological Phenomena::Child Nutritional Physiological Phenomena::Infant Nutritional Physiological Phenomena::Breast Feeding [Medical Subject Headings]lcsh:Science:Chemicals and Drugs::Nucleic Acids Nucleotides and Nucleosides::Nucleic Acids::RNA::RNA Bacterial [Medical Subject Headings]BifidobacteriumMultidisciplinaryBifidobacterium brevebiologyLacticaseibacillus rhamnosusMicrobiotaHibridación in SituInterleukin-10:Organisms::Bacteria::Gram-Positive Bacteria::Lactobacillales::Lactobacillaceae::Lactobacillus [Medical Subject Headings]Breast FeedingBloodCytokinesFemaleResearch ArticleAdult:Anatomy::Fluids and Secretions::Feces [Medical Subject Headings]Lactobacillus paracasei:Organisms::Bacteria::Endospore-Forming Bacteria::Gram-Positive Endospore-Forming Bacteria::Gram-Positive Endospore-Forming Rods::Clostridium::Clostridium difficile [Medical Subject Headings]Microbiology:Chemicals and Drugs::Nucleic Acids Nucleotides and Nucleosides::Nucleic Acids::Nucleic Acid Probes::Oligonucleotide Probes [Medical Subject Headings]Double-Blind MethodLactobacillus rhamnosusHumansImmunologic Factors:Analytical Diagnostic and Therapeutic Techniques and Equipment::Diagnosis::Diagnostic Techniques and Procedures::Clinical Laboratory Techniques::Cytological Techniques::Histocytological Preparation Techniques::Staining and Labeling::In Situ Hybridization [Medical Subject Headings]FecesSafety studiesved/biologyProbioticslcsh:RClostridium difficile:Organisms::Bacteria::Gram-Positive Bacteria::Actinobacteria::Bifidobacterium [Medical Subject Headings]biology.organism_classificationImmunoglobulin ALactobacilluslcsh:QInterleukin-4BifidobacteriumBreast feedingSondas de OligonucleótidosPLoS ONE
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A Cytologic Assay for Diagnosis of Food Hypersensitivity in Patients With Irritable Bowel Syndrome

2010

BACKGROUND & AIMS: A percentage of patients with symptoms of irritable bowel syndrome (IBS) suffer from food hypersensitivity (FH) and improve on a food-elimination diet. No assays have satisfactory levels of sensitivity for identifying patients with FH. We evaluated the efficacy of an in vitro basophil activation assay in the diagnosis of FH in IBS-like patients. METHODS: Blood samples were collected from 120 consecutive patients diagnosed with IBS according to Rome II criteria. We analyzed in vitro activation of basophils by food allergens (based on levels of CD63 expression), as well as total and food-specific immunoglobulin (Ig)E levels in serum. Effects of elimination diets and dou…

AdultMalefood hypersensitivitySettore MED/09 - Medicina InternaAdolescentCytologic assay; food hypersensitivity; irritable bowel diseaseCytological Techniquesirritable bowel diseasePlatelet Membrane GlycoproteinsImmunoglobulin ESensitivity and SpecificityIrritable Bowel SyndromeYoung AdultAntigenAntigens CDElimination dietmedicineAnimalsHumansYoung adultCells CulturedIrritable bowel syndromeCytologic assayHepatologybiologyCD63Tetraspanin 30business.industryGastroenterologyAllergensImmunoglobulin EMiddle Agedmedicine.diseaseBasophilsBasophil activationImmunologybiology.proteinFemaleAntibodybusinessClinical Gastroenterology and Hepatology
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Quantitatively characterizing drug-induced arrhythmic contractile motions of human stem cell-derived cardiomyocytes.

2018

Quantification of abnormal contractile motions of cardiac tissue has been a noteworthy challenge and significant limitation in assessing and classifying the drug-induced arrhythmias (i.e. Torsades de pointes). To overcome these challenges, researchers have taken advantage of computational image processing tools to measure contractile motion from cardiomyocytes derived from human induced pluripotent stem cells (hiPSC-CMs). However, the amplitude and frequency analysis of contractile motion waveforms doesn't produce sufficient information to objectively classify the degree of variations between two or more sets of cardiac contractile motions. In this paper, we generated contractile motion dat…

0301 basic medicineComputer scienceImage ProcessingComputational algorithmArrhythmiasRegenerative MedicineCardiovascularApplied Microbiology and Biotechnologyphase space reconstruction0302 clinical medicineComputer-AssistedImage Processing Computer-AssistedMyocytes CardiacComputingMilieux_MISCELLANEOUS[ INFO.INFO-IM ] Computer Science [cs]/Medical ImagingStem Cell Research - Induced Pluripotent Stem Cell - HumanOptical ImagingHeart DiseaseNetworking and Information Technology R&DStem cellBiological systemCardiacBiotechnologyCytological TechniquesInduced Pluripotent Stem CellsOptical flowTorsades de pointesImage processingBioengineeringarrhythmiaArticlebiosignal processingoptical flow03 medical and health sciencesMotionMatch movingmedicine[INFO.INFO-IM]Computer Science [cs]/Medical ImagingHumansMyocytesStem Cell Research - Induced Pluripotent Stem CellCardiac arrhythmiaArrhythmias CardiacTissue physiologymedicine.diseaseStem Cell ResearchMyocardial Contractioncardiac motion030104 developmental biology030217 neurology & neurosurgerySoftware
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Tumor mutational burden on cytological samples: A pilot study.

2020

Background Immune-checkpoint inhibitors (ICIs) represent an important treatment option for patients who have advanced stage non-small cell lung cancer (NSCLC). Currently, evaluation of the expression level of programmed death-ligand 1 (PD-L1) has proven highly successful as a positive predictive biomarker for ICIs. In addition to PD-L1, other promising predictive biomarkers are emerging, including high tumor mutational burden (TMB-H). However, measuring TMB-H remains challenging for several reasons, among which is the difficulty in obtaining adequate tissue material from NSCLC patients. There are no data in the current literature regarding the possibility of adopting cell blocks (CBs) for T…

OncologyMaleCancer Researchmedicine.medical_specialtyLung NeoplasmsCytological TechniquesDNA Mutational Analysis030209 endocrinology & metabolismPilot Projects03 medical and health sciences0302 clinical medicineInternal medicineCarcinoma Non-Small-Cell LungmedicineBiomarkers TumorHumansLung cancerPredictive biomarkerAgedRetrospective Studiesnext generation sequencingTMBbusiness.industryAdvanced stageTreatment optionsHigh-Throughput Nucleotide SequencingIon semiconductor sequencingAmpliconmedicine.diseasePrognosislung cancerOncology030220 oncology & carcinogenesisMutationcytologyTissue materialFemaleimmunotherapyNon small cellbusinessFollow-Up StudiesCancer cytopathologyReferences
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